Potential Liabilities of Gentamicin Homogeneous Enzyme Immunoassay
نویسندگان
چکیده
منابع مشابه
Potential liabilities of gentamicin homogeneous enzyme immunoassay.
We report two potential liabilities of the homogeneous enzyme immunoassay for the determination of serum gentamicin. One is a considerable loss of precision and accuracy at the ends of the calibration curve, and the other is an apparent loss of gentamicin with storage at -60 degrees C.
متن کاملEnzyme immunoassay for gentamicin.
We describe a gentamicin assay in which a peroxidase-gentamicin conjugate competes with gentamicin for binding to a gentamicin antibody adsorbed to a polystyrene solid phase. The assay can be completed in 30 min and requires 50 microliter of diluted serum. The precision and accuracy are equivalent to that of a radioimmunoassay technique and the reagents are stable for several months.
متن کاملNon-equilibrium enzyme immunoassay of gentamicin.
We describe a fast, accurate non-equilibrium enzyme immunoassay for serum gentamicin. With use of isokinetic conditions an assay takes a total of 12 min, including regeneration of the immunosorbent. The coefficient of correlation between the present method and conventional microbiological assays was 0.98.
متن کاملRapid, reproducible enzyme immunoassay for gentamicin.
An enzyme immunoassay for gentamicin utilizing glucose-6-phosphate dehydrogenase was compared to the radioimmunoassay. The enzyme immunoassay for gentamicin was accurate, specific, and easily performed. It offers an ulternative method to assay aminoglycosides and could be used in institutions using enzyme immunoassay to assay other drugs.
متن کاملModeling of homogeneous cloned enzyme donor immunoassay.
One of the most widely used analytical techniques for sensitive detection of biologically and clinically significant analytes is the immunoassay. In recent years direct immunoprobes allowing label-free detection of the interaction between the antibody and the target analyte have proved their capabilities as fast, simple, and nevertheless highly sensitive methods. Cloned enzyme donor immunoassay...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1982
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.21.1.192